I retook this photograph today using different camera settings. Today I increased the ISO to 800 and stopped down the aperture to 2.8 (was 1.8) yesterday. I was shooting in aperture priority mode and set the exposure to -1.0. I think this may have solved the sharpness issue. I believe it was a combination of camera shake, optimal lens aperture, and the autofocus on the lens being off my a few millimeters.
Here is a 100% crop of yesterday's image at the focal point:
And here is today's image at 100% crop:
I was obviously closer to the berries yesterday, but it didn't require nearly as much sharpening to produce the newer image. Yesterday's image was sharpened both in Camera RAW and again with unsharp mask. I think it obviously suffers for that and the noise explodes off of the photograph.
Hopefully I've managed to learn one of the limitations of my equipment. Now I just have to try to keep it in the back of my mind as I shoot in the future.
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